Instrument screening method for immunoassay is which?

Instrument screening method for immunoassay is which?

• A. Enzyme/fluoroimmunoassay
• B. Gas Chromatography
• C. Mass Spectrometry
• D. Thin Layer Chromatography

Answer: (A)

Immunoassays detect the target by antibody binding, and the readout is produced by a label that creates a measurable signal. The instrument-screened method for this type of assay is the enzyme/fluoroimmunoassay, which uses an enzyme reaction or a fluorescent label to generate a detectable signal read by an instrument. Gas chromatography, mass spectrometry, and thin-layer chromatography are separation/identification techniques and do not rely on antibody–antigen signaling, so they aren’t immunoassay screening methods.

Immunoassays detect the target by antibody binding, and the readout is produced by a label that creates a measurable signal. The instrument-screened method for this type of assay is the enzyme/fluoroimmunoassay, which uses an enzyme reaction or a fluorescent label to generate a detectable signal read by an instrument. Gas chromatography, mass spectrometry, and thin-layer chromatography are separation/identification techniques and do not rely on antibody–antigen signaling, so they aren’t immunoassay screening methods.